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goldengate genotyping assay  (GoldenGate Software Inc)

 
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    GoldenGate Software Inc goldengate genotyping assay
    Goldengate Genotyping Assay, supplied by GoldenGate Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goldengate+genotyping+assay/goldengate+genotyping+assay/10__1007_slash_s42161___024___01815___9-82-0-0
    Average 90 stars, based on 1 article reviews
    goldengate genotyping assay - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Variants in vincristine pharmacodynamic genes involved in neurotoxicity at induction phase in the therapy of pediatric acute lymphoblastic leukemia.
    Article Snippet: Vincristine is an important drug of acute lymphoblastic leukemia (ALL) treatment protocols that can cause neurotoxicity.. Patients treated with LAL/SHOP protocols often suffer from vincristine-related neurotoxicity in early phases of treatment.. A genetic variant in CEP72, a gene involved in vincristine pharmacodynamics, was recently associated with neurotoxicity after prolonged vincristine treatment.

    Article Title: Construction of a core collection of eggplant ( Solanum melongena L.) based on genome-wide SNP and SSR genotypes
    Article Snippet: By performing the GoldenGate genotyping assay with the DNA samples of the 938 accessions, the data of SNPs were obtained.

    Article Title: Genotyping and population characteristics of the China Kadoorie Biobank.
    Article Snippet: Genotyping was performed for a total of 1,040 plates according to GoldenGate Genotyping Assay Manual Protocols,88 with beadchip imaging using an iScan System (Illumina).

    Article Title: The pathogenicity and genetic diversity of the Indonesian blast pathogen from wide host ranges of rice sub-species
    Article Snippet: 1 Research Center for Horticulture, National Research and Innovation Agency, Jl.. Raya JakartaBogor, Cibinong, Bogor Regency, Indonesia 2 Research Center for Estate Crops, National Research and Innovation Agency, Jl.. Raya JakartaBogor, Cibinong, Bogor Regency, Indonesia 3 Research Center for Food Crops, National Research and Innovation Agency, Jl.

    Article Title: A Genome-Wide Study of Single-Nucleotide Polymorphisms in MicroRNAs and Further In Silico Analysis Reveals Their Putative Role in Susceptibility to Late-Onset Alzheimer's Disease.
    Article Snippet: Late-onset Alzheimer’s disease (LOAD) is a neurodegenerative disorder of growing relevance in an aging society for which predictive biomarkers are needed.. Many genes involved in LOAD are tightly controlled bymicroRNAs (miRNAs), which can be modulated by single-nucleotide polymorphisms (SNPs).. Our aim was to determine the association between SNPs in miRNAs and LOAD.We selected all SNPs in pre-miRNAs with a minor allele frequency (MAF) > 1% and genotyped them in a cohort of 229 individuals diagnosed with LOAD and 237 unrelated healthy controls.

    Article Title: Genetic Variation in Alcohol Dehydrogenase is Associated with Neurocognition in Men with HIV and History of Alcohol Use Disorder: Preliminary Findings
    Article Snippet: ADH SNPs were assayed using an array designed by NIAAA focused on SNPs with relevance to addictions ( Hodgkinson et al , 2008 ), using the Illumina BeadStation genotyping platform and GoldenGate genotyping assay.

    Article Title: A Clinical-Genetic Score for Predicting Weight Loss after Bariatric Surgery: The OBEGEN Study
    Article Snippet: DNA was extracted from saliva samples and processed by GoldenGate ® Genotyping Assay for VeraCode.

    Article Title: Genome-Wide Association Study Reveals Candidate Genes for Flowering Time in Cowpea ( Vigna unguiculata [L.] Walp.)
    Article Snippet: Previous cowpea genetic diversity study using a GoldenGate genotyping assay consisting of 1,536 single nucleotide polymorphisms (SNPs) on 442 cowpea landraces revealed the presence of two major gene pools in cultivated cowpea in Africa ( ).



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    Examples of SNP graphs observed in Lolium oligo pool assay (LOPA1) GoldenGate <t>genotyping.</t> SNP graphs are illustrated using the Software Illumina® GenomeStudio, version 2009.2. The normalized R (y-axis) is the normalized sum of intensities of the two dyes (Cy3 and Cy5), the normalized Theta (x-axis) is the deviation of Cy3 and Cy5 fluorescence from pure Cy3 and pure Cy5 signal (0 and 1). A normalized Theta value close to 0 and 1 is homozygous for SNP variant 1 and 2, respectively, a heterozygous sample is in between. The red, blue and purple ovals have the diameter of two standard deviations computed from the dispersal of the red, blue and purple dots, respectively. The numbers of plants in each cluster are indicated below the x-axis. ( A ) The 192 samples genotyped for SNP marker PTA.1021.C1 revealed fluorescence signal intensities close to 0, indicating assay failure. ( B ) Although the clustering algorithm at SNP PTA.1.C3 distinguished the three clusters at a GenTrain score of 0.40, such a genotyping pattern was considered inaccurate and this SNP was discarded from further analysis. ( C ) This illustration shows the SNP graph of monomorphic P9G02. ( D ) and ( E ) illustrate dominant SNPs being homozygous in one and heterozygous in the other mapping parent. For genetic linkage mapping, the markers PTA.109.C1 and PTA.291.C1 followed the segregation type nnxnp and lmxll, respectively . Dots corresponding to the parents of the VrnA mapping population (which are represented in duplicates) are highlighted in yellow. Graph ( F ) shows a classical example of a SNP marker being heterozygous in both parents following the segregation pattern hkxhk.
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    Image Search Results


    Examples of SNP graphs observed in Lolium oligo pool assay (LOPA1) GoldenGate genotyping. SNP graphs are illustrated using the Software Illumina® GenomeStudio, version 2009.2. The normalized R (y-axis) is the normalized sum of intensities of the two dyes (Cy3 and Cy5), the normalized Theta (x-axis) is the deviation of Cy3 and Cy5 fluorescence from pure Cy3 and pure Cy5 signal (0 and 1). A normalized Theta value close to 0 and 1 is homozygous for SNP variant 1 and 2, respectively, a heterozygous sample is in between. The red, blue and purple ovals have the diameter of two standard deviations computed from the dispersal of the red, blue and purple dots, respectively. The numbers of plants in each cluster are indicated below the x-axis. ( A ) The 192 samples genotyped for SNP marker PTA.1021.C1 revealed fluorescence signal intensities close to 0, indicating assay failure. ( B ) Although the clustering algorithm at SNP PTA.1.C3 distinguished the three clusters at a GenTrain score of 0.40, such a genotyping pattern was considered inaccurate and this SNP was discarded from further analysis. ( C ) This illustration shows the SNP graph of monomorphic P9G02. ( D ) and ( E ) illustrate dominant SNPs being homozygous in one and heterozygous in the other mapping parent. For genetic linkage mapping, the markers PTA.109.C1 and PTA.291.C1 followed the segregation type nnxnp and lmxll, respectively . Dots corresponding to the parents of the VrnA mapping population (which are represented in duplicates) are highlighted in yellow. Graph ( F ) shows a classical example of a SNP marker being heterozygous in both parents following the segregation pattern hkxhk.

    Journal: BMC Genomics

    Article Title: A transcriptome map of perennial ryegrass ( Lolium perenne L.)

    doi: 10.1186/1471-2164-13-140

    Figure Lengend Snippet: Examples of SNP graphs observed in Lolium oligo pool assay (LOPA1) GoldenGate genotyping. SNP graphs are illustrated using the Software Illumina® GenomeStudio, version 2009.2. The normalized R (y-axis) is the normalized sum of intensities of the two dyes (Cy3 and Cy5), the normalized Theta (x-axis) is the deviation of Cy3 and Cy5 fluorescence from pure Cy3 and pure Cy5 signal (0 and 1). A normalized Theta value close to 0 and 1 is homozygous for SNP variant 1 and 2, respectively, a heterozygous sample is in between. The red, blue and purple ovals have the diameter of two standard deviations computed from the dispersal of the red, blue and purple dots, respectively. The numbers of plants in each cluster are indicated below the x-axis. ( A ) The 192 samples genotyped for SNP marker PTA.1021.C1 revealed fluorescence signal intensities close to 0, indicating assay failure. ( B ) Although the clustering algorithm at SNP PTA.1.C3 distinguished the three clusters at a GenTrain score of 0.40, such a genotyping pattern was considered inaccurate and this SNP was discarded from further analysis. ( C ) This illustration shows the SNP graph of monomorphic P9G02. ( D ) and ( E ) illustrate dominant SNPs being homozygous in one and heterozygous in the other mapping parent. For genetic linkage mapping, the markers PTA.109.C1 and PTA.291.C1 followed the segregation type nnxnp and lmxll, respectively . Dots corresponding to the parents of the VrnA mapping population (which are represented in duplicates) are highlighted in yellow. Graph ( F ) shows a classical example of a SNP marker being heterozygous in both parents following the segregation pattern hkxhk.

    Article Snippet: Here, we present an efficient approach of using next generation sequencing (NGS) data for SNP discovery, and the successful design of a 768-plex Illumina GoldenGate genotyping assay in a complex genome.

    Techniques: Pool Assay, Software, Fluorescence, Variant Assay, Marker